Open AccessAn RNA‐Dependent Nucleoside Triphosphate Hydrolase from Krebs‐II Ascites Tumor Cells
Author(s) -
CHUMAKOV Konstantin M.,
ROZANOV Mikhail N.,
AGOL Vadim I.
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06871.x
Subject(s) - ascites , nucleoside , nucleoside triphosphate , chemistry , hydrolase , rna , biochemistry , adenosine triphosphate , enzyme , nucleotide , medicine , gene
A novel enzymatic activity, RNA‐dependent, NTPase, was isolated from Krebs‐II ascites tumor cells. This activity is associated with ribosomes and can be detached from them by washing in KCl solutions of a higher than 0.3 M concentration. The enzyme hydrolyzes all the four nucleoside triphosphates to the corresponding nucleoside diphosphates and orthophosphate. The rate of NTP hydrolysis increases about 10‐fold in the presence of natural RNAs and synthetic polyribonucleotides [except poly(G)]. Natural DNAs, both double and single‐stranded, are poor cofactors, although pol(dA) and poly(dT) stimulate, to a certain extent, the rate of ATP hydrolysis. Possible involvement of RNA‐dependent NTPase in protein biosynthesis is discussed.