Open AccessNucleotide Sequence of Cloned cDNA Coding for Mouse ɛ Casein
Author(s) -
HENNIGHAUSEN Lothar G.,
STEUDLE Annelotte,
SIPPEL Albrecht E.
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06818.x
Subject(s) - complementary dna , signal peptide , peptide sequence , coding region , nucleic acid sequence , biology , microbiology and biotechnology , casein , homology (biology) , protein sequencing , amino acid , sequence (biology) , nucleotide , biochemistry , gene
We isolated cDNA clones, which correspond to the mRNA coding for the smallest of the seven mouse caseins. From the nucleotide sequence of the cDNA we deduced the amino acid sequence of the protein, which we named ɛ casein. Mouse ɛ casein and cow α s2 casein show amino acid sequence homologies in the N‐terminal region of the mature protein. The signal peptide of mouse ɛ casein shows in length and sequence remarkable homology to the signal peptides of the calcium‐precipitable caseins of other species. In accordance with this group of caseins mouse ɛ casein contains in the sequence‐Ser‐Ser‐Glu‐Glu‐ a site for potential multiple phosphorylation.