Open AccessThe FI ‐Gene Product of Bacteriophage
Author(s) -
BENCHIMOL Sam,
BECKER Andrew
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06770.x
Subject(s) - isoelectric point , bacteriophage , gene product , lambda , gene , molecular mass , chemistry , microbiology and biotechnology , isoelectric focusing , stokes radius , biology , biochemistry , escherichia coli , physics , gene expression , enzyme , optics , size exclusion chromatography
The FI gene product of bacteriophage lambda has been purified extensively using a biochemical assay that measures assembly of lambda phage particles in vitro. The molecular weight of the native protein was estimated to be 21700 with an s 20,w of 2.1 S and a Stokes radius of 2.5 nm. The molecular weight in dodecylsulfate was estimated to be 19000. The protein is highly acidic with an isoelectric point less than 4.1.