Open AccessLocation and Nucleotide Sequence of frdB , the Gene Coding for the Iron‐Sulphur Protein Subunit of the Fumarate Reductase of Escherichia coli
Author(s) -
COLE Stewart T.,
GRUNDSTRÖM Thomas,
JAURIN Bengtåke,
ROBINSON John J.,
WEINER Joel H.
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06768.x
Subject(s) - escherichia coli , gene , protein subunit , coding region , sequence (biology) , nucleic acid sequence , genetics , nucleotide , biology , biochemistry , chemistry , microbiology and biotechnology
The frdB gene, encoding the iron‐sulphur protein subunit of fumarate reductase, has been located and its complete nucleotide sequence determined. The identity of the gene was confirmed by protein chemical studies and determination of the NH 2 ‐terminal sequence of the FrdB protein. The frdB gene is situated distal to and partially overlapped by frdA which codes for the flavoprotein subunit of the reductase. Its reading frame contains 244 codons and predicts a protein of M r 27092. In composition, the FrdB protein is strikingly similar to the corresponding subunit of the related flavoenzyme, succinate dehydrogenase. Analysis of the protein's primary structure revealed several features characteristic of iron‐sulphur proteins.