Open AccessComplexity of Polysomal Polyadenylated RNAs in Vicia faba Meristematic Root Cells
Author(s) -
BUFFARD Dominique,
VAILLANT Victor,
ESNAULT Robert
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06756.x
Subject(s) - vicia faba , meristem , polyadenylation , microbiology and biotechnology , biology , vicia , botany , rna , biochemistry , gene , shoot
As a first step in quantifying gene expression during differentiation of the Vicia faba root cells we have analysed the mRNA population from meristematic cells. Using cDNA · mRNA hybridizations we have shown that this population can be divided into three abundance classes (abundant, intermediate and rate) representing 37%, 34% and 29% of the mRNAs and containing 26, 610 and 11700 sequences respectively. The total base‐sequence complexity of the mRNA population, as determined by cDNA · mRNA hybridization, was found to be 1.6 × 10 7 nucleotides. This estimate was confirmed by the determination of the amount of single‐copy genomic DNA hybridizing to the mRNA. Expressed as the number of structural gene transcripts, this complexity corresponds to about 13000 mRNA sequences.