Open AccessIncorporation of Labelled Leucine into Alkaline Phosphatase in Response to 1,25‐Dihydroxycholecalciferol in Chick Intestinal Brush Borders
Author(s) -
WILSON Peter W.,
LAWSON D. Eric M.
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06718.x
Subject(s) - brush border , alkaline phosphatase , leucine , enzyme , phosphatase , biochemistry , chemistry , biology , membrane , amino acid , vesicle
1,25‐Dihydroxycholecalciferol increases the incorporation of leucine into a protein component ( M r = 84000) of the chick intestinal brush‐border membrane. Evidence has now been obtained showing this protein to be sub‐unit of alkaline phosphatase. Pure chick intestinal alkaline phosphatase was prepared and antibodies raised against it in rabbits. This enzyme and the brush‐border protein behaved identically on gel electrophoresis, and in terms of their phosphorylating activity and enzyme activity whether as the enzymically active dimeric form or when reduced to the sub‐unit form. The anti‐(alkaline phosphatase) reacted with the protein into which the incorporation of leucine was stimulated by 1,25‐dihydroxycholecalciferol.