Open AccessSubcellular Localization of the Methylcitric‐Acid‐Cycle Enzymes in Propionate Metabolism of Yarrowia Zipolytica
Author(s) -
UCHIYAMA Hiroo,
ANDO Masayasu,
TOYONAKA Yohko,
TABUCHI Takeshi
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06713.x
Subject(s) - yarrowia , biochemistry , peroxisome , enzyme , subcellular localization , propionate , mitochondrion , citric acid cycle , biology , metabolism , catabolism , lyase , cytoplasm , chemistry , yeast , gene
The subcellular localization of the four characteristic enzymes of the methylcitric acid cycle was studied with glucose‐grown as well as n‐alkane‐grown cells of Yarrowia lipolytica . Microsomes and peroxisomes showed no cycle enzyme activities. The four cycle enzymes were constitutively localized in mitochondria, with the exception of the dual localization of the fourth enzyme, 2‐methylisocitrate lyase, in mitochondria and cytoplasm, where the lyase may function to supply pyruvate (the end‐product of the catabolism of the propionate residue) to various reactions.