Open AccessCloning and Amplified Expression of the Tyrosyl‐tRNA Synthetase Genes of Bacillus stearothermophilus and Escherichia coli
Author(s) -
BARKER David G.
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06691.x
Subject(s) - escherichia coli , complementation , plasmid , pbr322 , biology , gene , microbiology and biotechnology , transformation (genetics) , recombinant dna , cloning (programming) , mutant , hybrid plasmid , transfer rna , biochemistry , rna , computer science , programming language
Two fragments of DNA which carry the genes coding for the tyrosyl‐tRNA synthetases of Escherichia coli and Bacillus stearothermophilus have been cloned into the plasmid pBR322 and were selected by complementation of an E. coli temperature‐sensitive mutant. Transformation of this strain with either of the recombinant plasmids results in a 100‐fold increase in tyrosyl‐tRNA synthetase activity measured in vitro and the protein products co‐migrate with the corresponding purified enzymes on polyacrylamide gels.