Open AccessTwo‐Dimensional NMR Spectroscopy of Siomycin A
Author(s) -
CLAYDEN Nigel J.,
INAGAKI Fuyuhiko,
WILLIAMS Robert J. P.,
MORRIS Gareth A.,
TORI Kazuo,
TOKURA Katsuya,
MIYAZAWA Tatsuo
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb06508.x
Subject(s) - chemical shift , proton , carbon fibers , nuclear magnetic resonance , spectroscopy , nuclear magnetic resonance spectroscopy , chemistry , aqueous solution , molecule , proton nmr , carbon 13 , analytical chemistry (journal) , materials science , physics , organic chemistry , nuclear physics , quantum mechanics , composite number , composite material
A trial application of a recent two‐dimensional nuclear magnetic resonance experiment to the polypeptide antibiotic siomycin A is described. Proton ‐ Carbon‐13 chemical shift correlation measures the proton and carbon‐13 chemical shift for cach directly bonded CH group in a molecule, in a single experiment. The resultant map of correlated chemical shifts enables the carbon‐13 spectrum to be assigned directly from the known proton shifts, and allows individual proton signals to be identified without problems of overlap. The signal‐to‐noise ratio available from such techniques should enable their application to aqueous protein solutions using currently available high‐field spectrometers.