Open AccessMembranes of Protein Bodies
Author(s) -
KARA Ursula A. K.,
KINDL Helmut
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb05819.x
Subject(s) - membrane , lysis , cucumis , sucrose , organelle , chemistry , biochemistry , chromatography , phospholipid , biology , botany
Protein bodies were prepared from cotyledons of germinating seeds of cucumber ( Cucumis sativus ) in different ways: the organelles either obtained from protoplasts by lysis or from cotyledons by mechanical disintegration were separated on sucrose‐density gradients. In addition, a non‐aqueous procedure was employed to isolate protein bodies. Marker proteins indicative of membranes of other organelles were carefully assayed. By this means contaminations in the purified protein‐body fractions could be ruled out. Isolated protein bodies were separated into crystalloids, matrix, and membranes. The membranes were purified and characterized according to their equilibrium density (Q = 1.20 kg/l) on sucrose gradients by flotation or sedimentation. Protein‐body inembranes labelled in the phospholipid moiety were prepared and analyzed after application of [ mrthyI −14C] choline or [ 32 P]phosphate in vivo .