Open AccessPurification, Properties and Assembly of the Neck‐Appendage Protein of the Bacillus subtilis Phage φ 29
Author(s) -
VILLANUEVA Nieves,
LÁZARO José M.,
SALAS Margarita
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb06365.x
Subject(s) - bacillus subtilis , appendage , in vitro , chemistry , biophysics , biochemistry , biology , microbiology and biotechnology , bacteria , anatomy , genetics
The purification of the neck appendage protein of φ29, p12*, which is involved in the adsorption of the phage to Bacillus subtilis , is described. The purified native protein is in a dimeric form and can be assembled, in vitro , onto purified 12 − particles that lack the neck appendages, suggesting that the incorporation of p12* to the rest of the phage structure is a self‐assembly process. The assembly of protein p12* in vitro follows cooperative kinetics and it occurs with an efficiency of about 4%.