Open AccessStructures of Fifteen Oligosaccharides Isolated from New‐Born Meconium
Author(s) -
MONTREUIL Jean,
STRECKER Gérard,
HERLANTPEERS MarieClaire,
DORLAND Lambertus,
HALBEEK Herman,
VELDINK Gerrit A.,
VLIEGENTHART Johannes F. G.
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb06336.x
Subject(s) - oligosaccharide , meconium , chemistry , mass spectrometry , glycoprotein , carbohydrate , nuclear magnetic resonance spectroscopy , catabolism , biochemistry , stereochemistry , methylation , chromatography , enzyme , biology , fetus , pregnancy , gene , genetics
New born meconium contains at least a hundred oTigosaccharides. In this study the isolation and characterization of the major constituents is described. The structure elucidation of 15 neutral and acidic oligosaccharides was carried out by methylation analysis, mass spectrometry and 360‐MHz 1 H‐NMR spectroscopy. The results show that the oligosaccharides accumulating in human meconium are probably products of the catabolism, of the O‐ and N‐linked carbohydrate chains of glycoproteins. It is proposed that endo‐ N ‐acetyl‐α‐ d ‐galactosaminidase, endo‐β‐ d ‐galactosidase and endo‐ N ‐acetyl‐β‐ d ‐glucosaminidase are involved in the production of these compounds.