Open AccessDecrease in the S1 Protein of 30‐S Ribosomal Subunits in Polyamine‐Requiring Mutants of Escherichia coli Grown in the Absence of Polyamines
Author(s) -
IGARASHI Kazuei,
KASHIWAGI Keiko,
KISHIDA Kazuo,
KAKEGAWA Tomohito,
HIROSE Seiyu
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb06182.x
Subject(s) - polyamine , mutant , escherichia coli , ribosomal protein , biochemistry , gel electrophoresis , protein subunit , polyacrylamide gel electrophoresis , chemistry , ribosomal rna , spermidine , microbiology and biotechnology , biology , enzyme , ribosome , gene , rna
The reason for the decrease of polypeptide‐synthetic activity of 30‐S ribosomal subunits obtained from two polyamine‐requiring mutants of Escherichia coli , grown in the absence of polyamines, has been studied by analyzing the total and split proteins of 30‐S subunits by disc gel and slab get electrophoreses. It was concluded that the decrease of S1 protein in 30‐S subunits was responsible for the decrease of polypeptide synthesis in polyamine‐requiring mutants of E. coli grown in the absence of polyamines.