Open AccessTranslation of Zea mays Endosperm Sucrose‐Synthase mRNA in vitro
Author(s) -
WÖSTEMEYER Johannes,
BEHRENS Ute,
MERCKELBACH Armin,
MÜLLER Markus,
STARLINGER Peter
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb06168.x
Subject(s) - endosperm , sucrose synthase , polysome , biochemistry , microbiology and biotechnology , messenger rna , reticulocyte , biology , polyacrylamide gel electrophoresis , enzyme , chemistry , rna , ribosome , gene , invertase
mRNA of 23‐day‐old maize endosperm was translated both in wheat germ extracts and rabbit reticulocyte lysates. A protein with an apparent molecular weight of 88000 comigrates in dodecylsulfate/polyacrylamide electrophoresis with sucrose synthase. This protein is precipitated with an antiserum against sucrose synthase and shows the same protease digestion pattern as the enzyme. It is not synthesized with mRNA extracted from sh/sh mutant kernels lacking sucrose synthase. By these criteria, the protein is the translation product in vitro of sucrose synthase mRNA. The separation of mRNA in methylmercury‐hydroxide‐agarose gels and subsequent translation indicates a length of sucrose synthase mRNA of 2800 nucleotides which is compatible with the coding length necessary for a protein with a molecular weight of 88000 plus untranslated sequences.