Open AccessFunctional Properties of Hemoglobins from Triturus cristatus
Author(s) -
CONDO Saverio G.,
GIARDINA Bruno,
LUNADEI Mario,
FERRACIN Alessandro,
BRUNORI Maurizio
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05707.x
Subject(s) - triturus , bohr effect , acclimatization , hemoglobin , bohr model , echinocyte , biology , chemistry , biophysics , biochemistry , zoology , ecology , physics , red blood cell , oxygen–haemoglobin dissociation curve , quantum mechanics
The functional properties of hemoglobins from Triturus cristatus carnifex have been characterized both from an equilibrium and a kinetic point of view. A special attention has been given to the effect of organic phosphates in view of their role in the modulation of the hemoglobin function. Under stripped conditions newt hemoglobins disply a negative Bohr effect which becomes much less pronounced in the presence of 0.2M inorganic phosphates. The effect of organic phosphates involves not only a drop in the O 2 affinity but also a large change in the shape of the O 2 Bohr effect which becomes markedly positive in the presence of myo inositol hexakis(phosphate). The experimental data reported make it possible to give a complete picture of the functional behaviour of newt hamoglobins, amking a contribution to clarifying the dependence of the sign of the Bohr effect on the acclimatization temperature of the animals that has been previously reported [Morpurgo, G., Battaglia, P.A. and Leggio, T. 1970) Nature(Lond). 225 76–77].