Open AccessSynthesis of Human Interferon β 1 in Escherichia coli Infected by a Lambda Phage Recombinant containing a Human Genomic Fragment
Author(s) -
MORY Yves,
CHERNAJOVSKY Yuti,
FEINSTEIN Sheldon I.,
CHEN Lousia,
NIR Uri,
WEISSENBACH Jean,
MALPIECE Yves,
TIOLLAIS Pierre,
MARKS David,
LADNER Martha,
COLBY Clarence,
REVEL Michel
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05689.x
Subject(s) - ecori , biology , escherichia coli , lambda phage , microbiology and biotechnology , recombinant dna , dna , genomic dna , genomic library , restriction map , restriction enzyme , gene , interferon , restriction fragment , insert (composites) , virology , plasmid , genetics , bacteriophage , base sequence , mechanical engineering , engineering
DNA from a human adult was fragmented by partial digestion with restriction endonuclease Eco RI and cloned in λ Charon 4A. Clone C15, with a human DNA insert of 17 × 10 3 bases, was identified as containg a gene for the fibroblast, interferon, interferon β 1 . Restriction mapping shows that this gene, located on a 1840‐base Eco RI fragment, is not interrupted by introns. Moreover, we show that this human genomic DNA fragment is able to direct the synthesis of active human interferon β 1 in Escherichia coli. Interferon activity of up to 7 × 10 6 U/1 was recovered from phage lysates by chromatography on CiBacron blue–Sepharose, and had the same immunological properties and species specificity as interferon produced by human fibroblasts.