Open AccessFormation of Leukotrienes E 3 , E 4 ,E 5 in Rat Basophilic Leukemia Cells
Author(s) -
ÖRNING Lars,
BERNSTRÖM Kerstin,
HAMMARSTRÖM Sven
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05667.x
Subject(s) - leukotriene c4 , leukotriene , chemistry , endogeny , leukotriene d4 , biochemistry , leukotriene e4 , arachidonic acid , basophilic , enzyme , biology , immunology , receptor , medicine , antagonist , asthma , pathology
Rat basophilic leukemia (RBL‐1) cells incubated with ionophore A23187 and 5,8,11‐eicosatrienoic acid produced three slow‐reacting substances identified as leukotrienes C 3 ,D 3 and E 3 by spectroscopic, chromatographic and enzymatic methods 5,8,11,14,17‐ Eicosapentaenoic acid was similarly converted by RBL‐1 cells to leukotrienes C 5 , D 5 and E 5 Leukotrienes C 4 ,D 4 and E 4 were also formed in these experiments from endogenous arachildonic acid Time‐course studies, incubations with 3 H‐labeled leukotriene C 3 and effects of acivicin [L‐(αS,5S)α amino‐3‐chloro 4,5‐dihydro‐5‐isoxazoleacetic acid; a γ‐glutamyl transpeptidase inhibitor] indicated that leukotrienes C and D are intermediates in the formation of leukotrienes E. L‐Cysteine enhanced the conversion of leukotriene C 3 to leukotriene D 3 and inhibited further degradation of leukotriene D 3 to leukotriene E 3 .