Open AccessThe Extent of Histone Acetylation Induced by Butyrate and the Turnover of Acetyl Groups Depends on the Nature of the Cell Line
Author(s) -
SCHRÖTER Hennrik,
GÓMEZLIRA M. Macarena,
PLANK KarinHeide,
BODE Jügern
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05664.x
Subject(s) - acetylation , butyrate , chinese hamster ovary cell , histone , microbiology and biotechnology , histone h4 , cell , cell culture , chemistry , biology , biochemistry , dna , genetics , fermentation , gene
Cells possessing widely different physiological and morphological features have been treated with substances known to stimulate the differentiation of erythroleukemia cells. Only short fatty acids are capable of causing a hyperacetylation of the core histones and of enhancing the level of an H1‐like protein in Chinese hamster ovary cells. While the time courses of a butyrate‐mediated acetylation are similar for all cells, the maximum histone acetyl contents are much higher for the transformed cell of a given type. A withdrawal of butyrate rapidly (within 45 min) gives rise to a ‘hypoacetylated state’ for fibroblasts and transformed fibroblast (epithelial) cells from which there is a slow recovery Lymphoid cells, on the other hand, display a marked persistence of the highly aaacetylated forms of histone H4.