Open AccessActivity of Phospholipase A 2 in the Inner Membrane of Rat‐Liver Mitochondria
Author(s) -
ZURINI Mauro,
HUGENTOBLER Gabriel,
GAZZOTTI Paolo
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05638.x
Subject(s) - inner membrane , phospholipase a2 , mitochondrion , phosphatidylethanolamine , inner mitochondrial membrane , phospholipase , membrane , biochemistry , enzyme , translocase of the inner membrane , chemistry , calmodulin , phospholipid , phosphatidylcholine , mitochondrial membrane transport protein
The inner membrane of rat liver mitochondria contains a highly active phospholipase A 2 which has alkaline pH optimum and requires Ca 2+ in the mocromolar range. The phospholipase is particularly active on the endogenous phosphatidylethanolamine and release relatively high amounts of docosahexanoic acid.The phospholipase A 2 of mitochondria or mitoplasts is not dependent on calmodulin. Using fluorescamine‐labelled mitoplasts there are indications that the enzyme is localized on both sides of the inner membrane.