Open AccessComparison of the Mannan Structure from the Cell‐Wall Mutant Candida sp. M‐7002 and Its Wild Type
Author(s) -
HAMADA Takashi,
NAKAJIMA Tasuka,
IZAKI Kazuo,
MATSUDA Kazuo
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05617.x
Subject(s) - mannan , mutant , mannose , biochemistry , cell wall , yeast , chemistry , wild type , galactose , side chain , polysaccharide , organic chemistry , gene , polymer
The cell‐wall mutant of a hydrocarbon‐assimilating yeast, Candida sp. M‐7002 and its wild type have shown a significant difference in mannose content. Each mannan was isolated from the mutant and the wild‐type cells by fractionation with Cetavlon and copper reagent. Both mannans contain d ‐mannose, d ‐glucose and phosphate. The mutant mannan has a relatively high content of protein (18% in weight bases) whereas the wild‐type mannan has a low protein content (5.1%) with a high amount of carbohydrate (<90%) Structural analyses by enzymatic and chemical methods showed that both mannans had an mannosidic(α1‐6)‐linked back bone substituted at O‐2 by side chains fo varying length. The side chains of the mutant mannan were shown to consist of single mannose units and disaccharide units whose linkages were predomonstrated that the mannans also contain mannosyl oligosaccharides linked to protein through O ‐glycosidic linkage. The chemical properties of the mannan of the Candida mutant indicates that the mutation might occur not only in the side chain structure but also in the (α1‐6)‐linked mannan back bone.