Open AccessMonoclonal Antibodies against Human Fibroblast Interferon
Author(s) -
HOCHKEPPEL HeinzKurt,
MENGE Ullrich,
COLLINS John
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05539.x
Subject(s) - monoclonal antibody , interferon , microbiology and biotechnology , fibroblast , antibody , polyacrylamide gel electrophoresis , clone (java method) , cell culture , biology , chemistry , alpha interferon , virology , biochemistry , enzyme , immunology , dna , genetics
A stable hybridoma clone derived by fusion of mouse myeloma cells (cell line Fo) and spleen cells of immunized mice has been isolated which secretes monoclonal antibodies against human fibroblast interferon (interferon‐β). The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon‐β separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and adsorbs interferon‐β immunologically when bound to CNBr‐activated Sepharose. It also inhibits the antiviral activity of human fibroblast interferon‐β from which the sugar moiety has been cleaved off by enzymatic treatment. The antibody is therefore probably directed against the protein moiety of the interferon molecules.