Open AccessPurification and Functional Properties of the Hemoglobin Components from the Rat (Wistar)
Author(s) -
CONDÒ Saverio G.,
GIARDINA Bruno,
BARRA Donatella,
GILL Stanley J.,
BRUNORI Maurizio
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05325.x
Subject(s) - hemoglobin , homogeneous , kinetics , chemistry , thermodynamics , ligand (biochemistry) , function (biology) , phase (matter) , biophysics , biochemistry , biology , receptor , physics , organic chemistry , genetics , quantum mechanics
Homogeneous components of Wistar rat hemoglobin have been isolated and characterized from the molecular and functional point of view. The O 2 equilibrium behaviour of the three main components (HbII, HbIII, HbIVA) has been investigated as a function of pH and organic phosphate concentration. The ligand‐binding kinetics of the isolated components have been also studied and are fully consistent with their equilibrium behaviour. It should be remarked that the choice of the system was governed largely by the ability of rat hemoglobins to crystallize very quickly. This almost unique molecular property together with the complete reversibility of the process may allow information to be obtained on the thermodynamics of ligand linked phase changes.