Open AccessEffect of Threonylcarbamoyl Modification (t 6 A) in Yeast tRNA Arg III on Codon‐Anticodon and Anticodon‐Anticodon Interactions
Author(s) -
WEISSENBACH Jean,
GROSJEAN Henri
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05320.x
Subject(s) - transfer rna , ribosome , polynucleotide , yeast , escherichia coli , nucleoside , biology , biochemistry , pseudouridine , threonine , chemistry , rna , enzyme , serine , gene
The effect of N ‐[9‐(β‐ d ‐ribofuranosyl) purin‐6‐ylcarbamoyl]threonine (t 6 A) adjacent to anticodon U * ‐C‐U of yeast tRNA Arg III (where U * is a modified U), compared to its unmodified adenosine counterpart, has been evaluated by three independent methods: (a) the polynucleotide‐directed binding of tRNA on ribosomes, (b) the ribosome‐free trinucleotide binding to the anticodon. (c) the anticodon‐anticodon binding test. The results obtained by these three methods indicate a small but significant stabilization effect of t 6 A on the binding of yeast tRNA Arg III with (a) poly(A,G) in the presence of Escherichia coli ribosomes, (b) free A‐G‐A triplet, and (c) E.coli tRNA Ser v (anticodon G‐G‐A). We therefore conclude that the stabilization effect of t 6 A occurs on U · A and U · G base pairs adjacent to the 5′ side of the modified nucleoside, most probably by stacking.