Open AccessThe Influence of Elongation‐Factor‐Tu · GTP and Anticodon‐Anticodon Interactions on the Anticodon Loop Conformation of Yeast tRNA Tyr
Author(s) -
WEYGANDDURASEVIC Ivana,
KRUSE Torben A.,
CLARK Brian F. C.
Publication year - 1981
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1981.tb05300.x
Subject(s) - ef tu , transfer rna , rnase p , gtp' , ternary complex , yeast , elongation factor , biochemistry , chemistry , rna , ribosome , enzyme , gene
The interactions of yeast tRNA Tyr , spin‐labelled at position i 6 A‐37 next to the anticodon, with EF‐Tu · GTP and with Escherichia coli tRNA Val (which has a complementary anticodon) have been studied. The immobilization of the spin label upon ternary complex formation shows a conformational change of the anticodon region, although this part of tRNA Tyr is not in direct contact with the protein, as indicated by RNase T 1 digestion. Upon anticodon‐anticodon interaction, no conformational change of the anticodon loop of tRNA Tyr was observed.