Open AccessA Histone‐Specific Acetyltransferase is Associated with Simian‐Virus‐40 Chromatin
Author(s) -
OTTO Bemd,
BÖHM Joachim,
KNIPPERS Rolf
Publication year - 1980
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1980.tb07213.x
Subject(s) - nucleoprotein , histone acetyltransferase , simian , chromatin , microbiology and biotechnology , biology , enzyme , sucrose gradient , histone , centrifugation , biochemistry , dna , chemistry
[ 14 C]Thymidine‐labeled simian virus 40 nucleoprotein. complexes were prepared from nuclei of lytically infected African green monkey kidney cells. The nucleoprotein complexes were further purified by sucrose gradient centrifugation and then incubated in the presence of [ 3 H]acetyl‐coenzyme A. We observed a transfer of [ 3 H]acetyl groups to the endogenous histones H2B, H3 and H4. The enzyme was solubilized in the presence of 0.5 M NaCl and showed properties described for the DNA‐binding acetyltransferase (J. Böhm, E. J. Schlaeger and R. Knippers, preceding paper in this journal).