Open AccessStructural Studies on Shigella flexneri Serotype 6 Core Region
Author(s) -
KATZENELLENBOGEN Ewa,
ROMANOWSKA Elżbieta
Publication year - 1980
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1980.tb06157.x
Subject(s) - shigella flexneri , serotype , mutant , heptose , shigella , periodate , chemistry , core (optical fiber) , twist , microbiology and biotechnology , biology , stereochemistry , biochemistry , escherichia coli , physics , gene , mathematics , geometry , optics
The core structure of Shigella flexneri serotype 6 has been investigated. The methods used in these studies involved methylation analysis, periodate oxidation, Smith degradation and enzymic modifications of core oligosaccharides isolated from two smooth strains and four rough mutants. As a result the complete core region was established:where d Gal p = d ‐galactopyranose, d Glc p = d ‐glucopyranose, l α d Hep p = l ‐ glycero ‐ d ‐ manno heptapyranose, dOclA = 3‐deoxy‐ d ‐ manno ‐octulosonic acid, d GlcN p = 2‐amino‐2‐deoxy‐ d ‐glucopyranose and P ‐ P EtN = pyrophosphorylethanolamine (partially substituted). In the R mutants examined two types of mutation have been observed: (1) defect in heptose region (lack of d ‐glucosamine) or (2) defects in hexose region of the core.