Open AccessPhotoinduced Protein‐RNA Cross‐Linking in Mammalian 80‐S Ribosomes
Author(s) -
REBOUD AnneMarie,
BUISSON Monique,
DUBOST Simone,
REBOUD JeanPaul
Publication year - 1980
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1980.tb05994.x
Subject(s) - ribosome , ribosomal rna , rna , protein subunit , eukaryotic ribosome , ribosomal protein , protein biosynthesis , biochemistry , eukaryotic large ribosomal subunit , chemistry , biophysics , biology , microbiology and biotechnology , gene
RNA‐protein interactions in the 80‐S rat liver ribosomes were studied by measuring cross‐linking of proteins to rRNAs induced by ultraviolet radiation, as already reported for free 40‐S and 60‐S subunits. Our results are compatible with the model in which most of the ribosomal proteins are accessible to rRNAs in the native conformational state of the ribosomes. Subunit association in 80‐S ribosomes does not seem to induce modifications in protein‐RNA interactions as measured by this irradiation technique. However, two proteins, S 9 and S 13 , appeared to be significantly less cross‐linked with RNA in ribosomes than in free subunits. Ribosomes which had been frozen and thawed several times were highly sensitive to ultraviolet radiation. Such treatment in the cold chiefly modified their 60‐S subunit moiety.