Open AccessPhoto‐Induced Crosslinking between Phenylalanine Transfer RNA and Messenger RNA on the Escherichia coli Ribosome
Author(s) -
MATZKE Antonius J. M.,
BARTA Andrea,
KUECHLER Ernst
Publication year - 1980
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1980.tb04998.x
Subject(s) - transfer rna , ribosome , rna , ribosomal rna , five prime cap , escherichia coli , chemistry , messenger rna , yeast , phenylalanine , biochemistry , non coding rna , amino acid , gene
Crosslinking between phenylalanyl transfer RNA from brewer's yeast and poly(U) can be obtained when ribosomal complexes are irradiated at > 300 nm; the ribosomal complexes were formed by enzymatic binding of the tRNA. The crosslinked product was isolated on oligo(dA)cellulose columns. The experiments suggest that the photo‐crosslinking occurs via the wybutine in the anticodon loop of the tRNA. This system can be used to study interactions between tRNA and messenger RNA on the ribosome.