Glycosylation of Influenza Virus Proteins in the Presence of Fluoroglucose Occurs via a Different Pathway

In the presence of fluoroglucose, an inhibitor of formation of mannosylphosphoryl and glucosyl‐phosphoryl‐dolichol, lipid‐dependent glycosylation of influenza virus glycoproteins is strongly, but not completely inhibited. The oligosaccharides that were transferred to protein in the presence of fluoroglucose came directly from dolichol‐linked intermediates. However, they were smaller than the normal high‐mannose oligosaccharides and, furthermore, resistant towards digestion with endo‐β‐ N ‐acetylglucosaminidase H. By excluding mannosylphosphoryl‐dolichol, similar dolichyl‐pyrophosphate‐linked intermediates were synthesized in vitro by membranes from fluoroglucose‐treated cells and they were shown to glycosylate protein.