Open AccessBovine Kidney Pyruvate Dehydrogenase Complex
Author(s) -
KRESZE GeorgB.,
RONFT Hedwig,
DIETL Brigitte
Publication year - 1980
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1980.tb04510.x
Subject(s) - pyruvate dehydrogenase complex , pyruvate dehydrogenase phosphatase , branched chain alpha keto acid dehydrogenase complex , dihydrolipoyl transacetylase , pyruvate dehydrogenase kinase , biochemistry , oxoglutarate dehydrogenase complex , pyruvate decarboxylation , pyruvate dehydrogenase lipoamide kinase isozyme 1 , papain , dehydrogenase , chemistry , enzyme , biology
1 Bovine kidney pyruvate dehydrogenase multienzyme complex is inactivated rapidly by papain. However, none of the component activities of the complex is destroyed during inactivation of the overall reaction. 2 The core component, lipoate acetyltransferase, is cleaved by papain to give principal fragments with M r 26500 and 26000 (as determined by dodecylsulfate gel electrophoresis). Much more slowly, the α chain of the pyruvate dehydrogenase component is attacked. 3 Fragmented lipoate acetyltransferase retains its complete enzymatic activity and remains of high molecular weight. It is unable, however, to bind the other component enzymes, pyruvate dehydrogenase and lipoamide dehydrogenase. Therefore, the multienzyme complex is disassembled when treated with papain. 4 A method is described which allows the rapid and convenient isolation of nicked lipoate acetyltransferase as well as unfragmented pyruvate dehydrogenase and lipoamide dehydrogenase from papain‐treated complex under very mild conditions. The two uncleaved component enzymes have identical properties and similar specific activities as enzyme preparations obtained by other, more laborious procedures.