Open AccessA DNA‐Binding Protein Specific for the Early Replicated Region of the Chromosome Obtained from Escherichia coli Membrane Fractions
Author(s) -
JACQ Annick,
KOHIYAMA Masamichi
Publication year - 1980
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1980.tb04470.x
Subject(s) - dna , escherichia coli , microbiology and biotechnology , biology , dna replication , seqa protein domain , biochemistry , single stranded binding protein , circular bacterial chromosome , chemistry , dna binding protein , origin of replication , gene , transcription factor
After extensive dialysis of Escherichia coli membranes treated with Triton X‐100, three membrane proteins (A, B and B′) with an affinity for DNA have been isolated and purified. They bind to either double‐stranded or single‐stranded DNA. A and B′ proteins preferentially attach to DNA even in the presence of poly(uridylic acid). Only protein B′ can recognize some base sequence of DNA because pulse‐labelled DNA made at the initiation of replication in a synchronized dnaC mutant has been selectively retained by the protein.