Open AccessThe Peroxidase Activity of Rat Uterus
Author(s) -
OLSEN Ragnar L.,
LITTLE Clive
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb19725.x
Subject(s) - peroxidase , enzyme , biochemistry , substrate (aquarium) , polyacrylamide gel electrophoresis , chemistry , polyacrylamide , uterus , biology , endocrinology , polymer chemistry , ecology
The peroxidase activity in the uterine extract of rats previously given a low dose of oestradiol has been examined. From sexually mature rats, two peroxidases of apparent molecular weights of 92000 (peroxidase I) and 40000 (peroxidase II) and of different mobilities in polyacrylamide discgel electrophoresis were found. The two peroxidases were separated and characterized in terms of their substrate specificities, kinetics, pH optima for activity, inhibitors and stabilities. Both enzymes appeared to be classic haemoprotein peroxidases of very similar properties. Peroxidase II was further purified.