Open AccessEffects of Apramycin, a Novel Aminoglycoside Antibiotic on Bacterial Protein Synthesis
Author(s) -
PERZYNSKI Stanislaw,
CAN Michael,
CUNDLIFFE Eric,
CHAHWALA Suresh B.,
DAVIES Julian
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13295.x
Subject(s) - bacillus megaterium , aminoglycoside , escherichia coli , in vivo , protein biosynthesis , chemistry , antibiotics , serine , bacteria , biochemistry , microbiology and biotechnology , biology , enzyme , genetics , gene
1. The novel aminoglycoside antibiotic apramycin is shown to be a potent inhibitor of protein synthesis in bacteria both invivo and invitro. 2. In cell‐free systems from Escherichia coli programmed with poly(U), apramycin induces translation errors, as assayed by incorporation of leucine, isoleucine and serine, although this effect occurs only to a limited extent. 3. Apramycin inhibits the translocation step of protein synthesis both invivo , in protoplasts of Bacillus megaterium , and invitro , in cell‐free systems from E. coli. It is proposed that this is the primary inhibitory effect of the drug.