Open AccessIsolation and Purification of a Neurodepressing Hormone from the Eyestalk of Procambarus bouvieri (Ortmann)
Author(s) -
HUBERMAN Alberto,
ARECHIGA Hugo,
CIMET Avivah,
ROSA Jorge,
ARAMBURO Carlos
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13246.x
Subject(s) - crayfish , sephadex , chromatography , chemistry , size exclusion chromatography , electrophoresis , eyestalk , biochemistry , hormone , biology , ecology , enzyme
1. A neurodepressing hormone has been isolated and purified to homogeneity from aqueous extracts of 2000 eyestalks of the Mexican crayfish Procambarus bouvieri (Ortmann). 2. Purification was achieved by gel filtration on Sephadex G‐25 and G‐15, and preparative paper electrophoresis at four pH values (1.8, 3.6, 6.0 and 10.0). 3. The recovery of hormone activity was 85% and the specific activity was 4.0 × 10 4 times that of the starting material. 4. The hormone is a thermostable peptide of approximately 1200 molecular weight and composed of neutral amino acids. 5. No N‐terminal group could be found. From its electrophoretic behavior it is concluded that the C‐terminal group is also blocked.