Open AccessSomatic Antigens of Shigella
Author(s) -
DMITRIEV Boris A.,
KNIREL Yurily A.,
SHEREMET Olga K.,
SHASHKOV Alexander A.,
KOCHETKOV Nikolay K.,
HOFMAN Irihna L.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13190.x
Subject(s) - chemistry , chromium trioxide , acid hydrolysis , polysaccharide , hydrolysis , sephadex , galactose , chromatography , biochemistry , periodate , organic chemistry , enzyme , chromium
The specific polysaccharide was obtained from the lipopolysaccharide of Shigella newcastle by mild acid hydrolysis and further purified by permeation chromatography on Sephadex G‐50. It was found to consist of l ‐rhamnose, 2‐acetamido‐2‐deoxy‐ d ‐galactose, d ‐galacturonic acid residues and O ‐acetyl groups in the molar ratios of 2:1:1:1. On the basis of 1 H and 13 C nuclear magnetic resonance spectroscopy, methylation analysis, partial acid hydrolysis, Smith degradation, and chromium trioxide oxidation, the following structure can be assigned to the repeating oligosaccharide unit of the polysaccharide:‐4) d GalA(β1‐3) d GalNAc‐(β1‐2) l Ac 3 Rha(α1‐2) l Rha(α1‐, where Gal A = galacturonic acid, GalNAc = N ‐acetylgalactosamine, Ac 3 Rha = 3‐ O ‐acetylrhamnose. The structural and immunochemical data presented prove that Sh. newcastle lipopolysaccharide belongs to a ‘non‐classical’ type of somatic antigens with acidic O‐specific polysaccharide chains.