Open AccessMitochondrial Protein‐Synthesizing Machinery in Saccharomyces cerevisiae Grown in Different Metabolic Conditions
Author(s) -
BALDACCI Giuseppe,
FALCONE Claudio,
FRANCISCI Silvia,
FRONTALI Laura,
PALLESCHI Claudio
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13175.x
Subject(s) - saccharomyces cerevisiae , respiration , serine , biology , biochemistry , mitochondrion , alanine , transfer rna , metabolism , gene , function (biology) , microbiology and biotechnology , amino acid , rna , enzyme , botany
1 The isoacceptor patterns of mitochondrial seryl and alanyl‐tRNAs from Saccharomyces cerevisiae grown under different conditions and committed to fermentative or respiratory metabolism have been studied by reversed‐phase chromatography. 2 An extensive variability of the chromatographic patterns of the four isoacceptors for serine and of the three isoacceptors for alanine has been observed as a function of carbon source and stage of growth, but the main differences were observed in the different stages of growth on glucose. 3 In order to distinguish the effects due to stage of growth from those due to relief from glucose repression, the isoacceptor patterns of mitochondrial tRNA were compared in repressed and derepressed resting cells; results show that some of the mitochondrial species are almost undetectable in resting repressed cells, but are the major ones after recovery of respiration. 4 Two of the serine isoacceptors (species 1 and 2), one of which is absent or not acylable in repressed resting cells, are different gene products.