Open AccessIsolation and Characterization of a Sulfated Glyceroglucolipid from Alveolar Lavage of Rabbit
Author(s) -
SLOMIANY Amalia,
SMITH Fred B.,
SLOMIANY Bronislaw L.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13158.x
Subject(s) - chromium trioxide , glycolipid , chemistry , chromatography , silicic acid , periodate , sulfation , sephadex , biochemistry , organic chemistry , chromium , enzyme
A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE‐Sephadex and silicic acid, and thin‐layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono‐ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO 3 H‐6Glcα1→6Glcαt1→6Glcα1→6Glcα1→3‐1,(3)‐ O ‐alkyl‐2‐ O ‐acylglycerol.