Open AccessRegions of the Lipopolysaccharide of Pseudomonas aeruginosa Essential for Antitumor and Interferon‐Inducing Activities
Author(s) -
TANAMOTO Kenichi,
ABE Chiyoji,
HOMMA J. Yuzuru,
KOJIMA Yasuhiko
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13152.x
Subject(s) - lipid a , lipopolysaccharide , in vitro , pseudomonas aeruginosa , interferon , polysaccharide , chemistry , microbiology and biotechnology , biochemistry , biology , bacteria , immunology , genetics
Resistance against ascites tumor development and interferon‐inducing activity were demonstrated in lipopolysaccharide derived from the protein‐lipopolysaccharide complex obtained from an autolysate of Pseudomonas aeruginosa. Lipid A obtained from the lipopolysaccharide was sufficient to induce interferon in vitro but no antitumor activity was found if lipid A or the polysaccharide derived from lipopolysaccharide was injected into the animal. Chemical modification of the polysaccharide portion or deacylation of the lipopolysaccharide also diminished antitumor activity. In contrast, interferon was induced by these incomplete lipopolysaccharides. These results indicate that both the lipid A portion and covalently linked polysaccharide are necessary for the inhibition of ascites tumor development, whereas incomplete lipid A with amide‐linked fatty acids is sufficient to induce interferon in vitro.