Open AccessTranslation in vitro of Tetrahymena pyriformis Polyadenylated mRNA
Author(s) -
PORTIER MarieMadeleine,
MILET Michele,
HAYES Donal H.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13098.x
Subject(s) - tetrahymena pyriformis , reticulocyte , polyadenylation , protein biosynthesis , rna , tetrahymena , ribosome , translation (biology) , biology , in vitro , messenger rna , cell free system , isoelectric focusing , microbiology and biotechnology , biochemistry , lysis , gene , enzyme
The capacity of poly(A)‐containing RNA of the protozoan ciliate Tetrahymena piriformis to direct the synthesis of proteins in vitro has been tested using two cell‐free systems: a wheat germ extract and a rabbit reticulocyte lysate. The results obtained with these two systems are compared and the identification of α and β tubulins among the products of protein synthesis in vitro, after separation by one‐dimensional and two‐dimensional electrophoresis, is described. By isoelectric focusing in polyacrylamide gels, each species of tubulin is resolved into several bands, suggesting that the main subunits are more heterogeneous than has been generally described. Poly(A)‐containing RNA has also been fractionated on a 70% formamide/sucrose gradient and it is shown that α and β tubulins are coded by separate mRNAs.