Open AccessBiochemical Properties and Localization of the Chromosomal Protein IP25
Author(s) -
KEPPEL France,
ALLET Bernard,
EISEN Harvey
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb13060.x
Subject(s) - micrococcal nuclease , chromatin , nucleosome , histone , digestion (alchemy) , biology , microbiology and biotechnology , chemistry , biophysics , biochemistry , dna , chromatography
The protein IP25, which has previously been reported to accumulate in the chromatin during erythroid differentiation of Friend‐virus‐transformed erythroleukemia cells (FL cells), is shown to behave like histone H1 without being structurally related to it. Like H1. IP25 is not released by digestion of FL cells nuclei with DNAse I. After micrococcal digestion IP25 and HI are differentially distributed in the nucleosome monomers and dimers. This distribution suggests an internucleosomal location for IP25 and H1. Different rates of digestion are observed between nuclei of differentiating and non‐differentiating FL cells with both DNAse I and micrococcal nuclease. These differences could be due to the presence of IP25 in the chromatin of differentiating cells.