Open AccessLipid Phase Transitions and Procoagulant Activity
Author(s) -
TANS Guido,
ZUTPHEN Henk,
COMFURIUS Paul,
HEMKER H. Coenraad,
ZWAAL Robert F. A.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb12984.x
Subject(s) - dipalmitoylphosphatidylcholine , prothrombinase , miscibility , chemistry , differential scanning calorimetry , phosphatidylserine , phospholipid , phosphatidylcholine , phase diagram , phase transition , phase (matter) , chromatography , liposome , analytical chemistry (journal) , crystallography , thermodynamics , organic chemistry , biochemistry , thrombin , polymer , membrane , platelet , physics , biology , immunology
1 The relation between acyl chain fluidity of synthetic phosphatidylserine/phosphatidylcholine mixtures and their activity in the prothrombinase complex has been investigated using differential scanning calorimetry and a one‐stage prothrombinase assay. 2 Mixtures of dioleoylphosphatidylserine and dioleoylphosphatidylcholine which are liquid crystalline at physiological temperature proved to be considerably more active in coagulation than mixtures of dipalmitoylphosphatidylserine and dipalmitoylphosphatidylclioline which are in the gel phase, while this difference disappears completely upon addition of cholesterol. Varying the proportion of phosphatidylserine from 30 to 70 mol/100 mol has no significant influence on the coagulant behaviour. 3 The procoagulant activity of mixtures composed of dimyristoylphosphatidylserine and dipalmitoylphosphatidylcholine was compared with the phase diagram constructed from the calorimetric data. Going through the phase transition to the liquid state was accompanied by a sharp increase of clot‐promoting activity. 4 The phospholipid concentration which exhibited maximal coagulant activity appeared to be strongly dependent on the miscibility properties of the lipids. Solid/solid immiscibility was observed both with mixtures of the two dimyristoyl compounds and the dipalmitoyl compounds, and a 5–10‐fold increase in the lipid concentration required for maximal coagulant activity was observed below the phase transition. This shift was found to be absent with mixtures of the dioleoyl species or with dimyristoylphosphatidylserine/dipalmitoylphosphatidylcholine both of which showed essentially complete miscibility in both phases.