Open AccessThe Reaction of Nitrogen Monoxide and of Nitrite with Deoxyhaemocyanin and Methaemocyanin of Helix pomatia
Author(s) -
VERPLAETSE Jozef,
TORNOUT Philippe,
DEFREYN Ghislain,
WITTERS Raphaël,
LONTIE René
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb12969.x
Subject(s) - electron paramagnetic resonance , chemistry , nitrite , helix pomatia , hyperfine structure , derivative (finance) , photochemistry , copper , carbon monoxide , nuclear magnetic resonance , crystallography , organic chemistry , catalysis , ecology , physics , quantum mechanics , snail , financial economics , nitrate , economics , biology
Deoxyhaemocyanin, treated with NO under strictly anaerobic conditions, yielded methaemocyanin and N 2 O in a fast reaction. In a further slow reaction this methaemocyanin lost its triplet electron paramagnetic resonance (EPR) signal at g = 4 and yielded a nitrosyl derivative with a characteristic g = 2 Cu(II) EPR signal, indicating the binding of a single NO per copper pair. Thus under strictly anaerobic conditions deoxyhaemocyanin and methaemocyanin, treated with NO, gave the same derivative as shown by circular dichroism and EPR spectra. Methaemocyanin yielded, moreover, reversibly a nitrite derivative, characterized by a triplet signal at g = 4 with 7 hyperfine lines.