Open AccessImmunochemical Studies of Partially Hydrolyzed Lipopolysaccharide from Fusobacterium nucleatum Fev1
Author(s) -
HOFSTAD Tor,
FREDRIKSEN Gjert
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb12871.x
Subject(s) - heptose , chemistry , fractionation , size exclusion chromatography , hydrolysis , fusobacterium nucleatum , chromatography , glucosamine , acid hydrolysis , lipid a , elution , polysaccharide , fraction (chemistry) , biochemistry , lipopolysaccharide , bacteria , biology , enzyme , genetics , porphyromonas gingivalis , mutant , gene , endocrinology
Fusobacterium nucleatum Fev1 lipopolysaccharide was split by hydrolysis with 1% acetic acid into acid‐soluble polysaccharide and lipid A. Gel filtration of the polysaccharide on Bio‐Gel P‐60 gave a high‐molecular‐weight fraction eluted with the void volume, and a fraction eluted at 2.4 × V 0 . The high‐molecular‐weight fraction contained L‐ glycero ‐D‐ manno ‐heptose in relatively large amounts, glucose, glucosamine, an unknown amino compound and small amounts of (or no) D‐ glycero ‐D‐ manno ‐heptose. Phosphorus and 3‐deoxy‐D‐ manno ‐octulosonic acid were not detected. The other fraction contained L‐ and D‐ glycero ‐D‐ manno ‐heptose, glucose, glucosamine, 3‐deoxy‐D‐ manno ‐octulosonic acid and phosphorus. Further fractionation experiments and serological investigations indicated that the high‐molecular‐weight fraction carried the O‐antigenic side chains, whereas the material eluted from Bio‐Gel P‐60 at 2.4 × V 0 represented the core oligosaccharide.