Open AccessCrystallization of Bacteriophage MS2
Author(s) -
MIN JOU Willy,
RAEYMAEKERS Alex,
FIERS Walter
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb04276.x
Subject(s) - crystallization , bacteriophage , crystallography , materials science , bacteriophage ms2 , ethylene glycol , chemistry , chemical physics , organic chemistry , biochemistry , escherichia coli , gene
Crystals of bacteriophage MS2 have been obtained by slowly cooling a 1% virus solution from 23°C to 0°C in the presence of poly(ethylene glycol) 6000. The crystals were colorless, needle‐like, anisotropic and very fragile. Electron microscopic observation of the crystals revealed a two‐dimensional lattice of particles with RNA phage morphology and dimensions. Preliminary X‐ray examination of the crystals confirmed their viral nature.