Open AccessThe Binding of 8‐Anilinonaphthalene‐1‐sulphonate to a Fixed Concentration of Unenergised and Succinate‐Energised Submitochondrial Particles
Author(s) -
GAINS Nigel,
DAWSON Alan P.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb04263.x
Subject(s) - submitochondrial particle , fluorescence , chemistry , biophysics , crystallography , mitochondrion , biochemistry , biology , physics , quantum mechanics
The effect of the approximately hyperbolic relationship between fluorochrome concentration and light absorbed on the interpretation of data for the binding of 8‐anilinonaphthalene‐1‐sulphonate to unenergised and succinate‐energised submitochondrial particles has been investigated. If this is taken into account plots of fluorescence against fluorescence × [fluorochrome] −1 do not tend towards a maximum fluorescence value. The significance of this findings is discussed.