Open AccessComparison of Poly(A)‐Containing RNAs in Different Cell Types of the Lower Eukaryote Schizophyllum commune
Author(s) -
ZANTINGE Bert,
DONS Hans,
WESSELS Joseph G. H.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb04238.x
Subject(s) - rna , schizophyllum commune , polyadenylation , nuclease protection assay , biology , dna , eukaryote , microbiology and biotechnology , nucleotide , messenger rna , non coding rna , mycelium , biochemistry , gene , botany , genome
Poly(A)‐containing RNAs were isolated from morphologically different cells of the fungus Schizophyllum commune . Using mRNA markers the number‐average length of poly(A)‐containing RNA in total RNA and in purified poly(A)‐containing RNA was estimated as 1100 nucleotides. Number‐average length of poly(A)‐tracts was 33 nucleotides. 2.5% of total RNA is poly(A)‐containing RNA and probably up to 7.5% are non‐polyadenylated polydisperse RNA sequences. Saturation hybridization of poly(A)‐containing RNA to gap‐translated [ 3 H]DNA resulted in 16% of the reactive single‐copy DNA to become S1 nuclease resistant. It was found that purified poly(A)‐containing RNA represented the entire RNA complexity, i. e. 10000 different RNA sequences in S . commune . RNA sequences isolated from morphologically different mycelia and from fruiting and non‐fruiting mycelia were identical for at least 90%.