Open AccessQualitative and Quantitative Changes in Uterine mRNA Populations in Response to Oestradiol Treatment of Rats
Author(s) -
AZIZ Shaharuddin,
BALMAIN Allen,
KNOWLER John T.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb02037.x
Subject(s) - messenger rna , in situ hybridization , endocrinology , population , rna , biology , medicine , hormone , andrology , genetics , gene , environmental health
The mRNA population of immature rat uteri, responding to different lengths of oestradiol‐induced differentiation, have been compared by the technique of DNA hybridization with heterogeneous RNA. Over the first 4 h of hormone induction there are continuing qualitative and quantitative changes in the poly(A)‐containing mRNA such that, by 4 h after oestradiol administration, the population bears little resemblance to that of the unstimulated animal. Between 2 h and 4 h after oestradiol administration, the most striking changes appear to be in sequences of intermediate abundance. The possible significance of these findings is discussed.