Open AccessDiversity and Complexity of Uterine mRNA from Rats of Differing Hormonal States
Author(s) -
AZIZ Shaharuddin,
BALMAIN Allen,
KNOWLER John T.
Publication year - 1979
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1979.tb02036.x
Subject(s) - diversity (politics) , messenger rna , hormone , microbiology and biotechnology , biology , chemistry , medicine , endocrinology , biochemistry , gene , political science , law
The diversity and complexity of the uterine mRNA population has been compared at two different stages of uterine growth and development in the rat. Analysis by cDNA hybridization to homologous mRNA indicates that there are 8000 different sequences expressed in the immature rat uterus responding to just 4 h of oestradiol‐17β‐induced growth while the fully developed uterus expresses 36000 sequences. As a check on our results, the complexity is re‐analysed by hybridization of mercurated mRNA to total unique DNA. Analysis by this method indicates that the hormone‐stimulated immature rat uterus contains 12000 poly(A)‐containing mRNA sequences while the fully differentiated adult tissue contains 53000 diverse sequences.