Nucleotide Sequence of the Hin d‐C Fragment of Simian Virus 40 DNA

We report here the nucleotide sequence of the wild‐type simian virus 40 (strain 776) restriction fragment Hin d‐C‐P 1 DNA and of the homologous region of various mutant DNAs which lack part of this fragment. During this work, we detected between Eco RII fragments N and G an additional, 17‐base‐pair Eco RII fragment, fragment P, which had previously been overlooked. Also, an additional dTpdG dinucleotide at residues L 339–340 was observed by sequence analysis of the DNA minus (E) strand; the presence of this dinucleotide was masked on sequencing patterns of the plus strand due to the persistence (during gel electrophoresis) of some secondary structures in the strand's 5′‐terminal region. These nucleotide additions raise the total length of SV40 DNA to 5243 base pairs. The longest tandemly repeated segment in SV40 DNA now extends over 72 base pairs. SV40 deletion mutants dl 893 and dl 894 and SV40 strains Rh 911 and 1802 all lack an identical 72‐base‐pair‐long DNA segment in the Hin d‐C region. This deletion corresponds precisely to one of the two aforementioned large tandemly repeated sequences. Mutant dl 895 lacks 66 base pairs, 63 of which are part of the former repetition. All these mutants, except dl 895, very probably were generated by an intramolecular, homologous recombination event. The 40‐base‐pair deletion in mutant dl 1811 includes the major capping site of SV40 late RNA. dl 1812 lacks only three base pairs, which are part of the overlapping Hha I and Hpa II restriction sites at position 0.725–0.726.