Open AccessStudies on Lipid A from Yersinia pseudotuberculosis Lipopolysaccharide
Author(s) -
KRASIKOVA In.,
GORBACH Vladimir I.,
SOLOV'EVA Tamara F.,
OVODOV Yury S.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb20925.x
Subject(s) - yersinia pseudotuberculosis , lipid a , residue (chemistry) , chemistry , fatty acid , hydrolysis , glucosamine , lipopolysaccharide , acid hydrolysis , biochemistry , acetic acid , myristic acid , organic chemistry , palmitic acid , biology , virulence , gene , endocrinology
Lipid A was isolated from lipopolysaccharide of Yersinia pseudotuberculosis S form (strain 341, subtype IB) using mild hydrolysis with acetic acid. The purified material (yield about 25%, molecular weight about 2900) contained d ‐glucosamine (11%), fatty acids (54%), protein concomitant (9.7%) and phosphorus (∼ 2%). Dodecanoic and 3‐hydroxy‐tetradecanoic acids in a molar ratio of 1:3.6 were detected as major fatty acid constituents. The hydroxyl groups of d ‐glucosamine were acylated with the residues of both fatty acids, while the amino groups were substituted with the residue of 3‐hydroxy‐tetradecanoic acid. Such a simple fatty acid composition is reminiscent of that found in lipid A in Y. pestis.